codon optimized vly sequence Search Results


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ATCC codon optimized vly sequence
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GenScript corporation recombinant vaginolysin vly
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LoveYourself Inc vly
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Cell Signaling Technology Inc vaginolysin
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New England Biolabs toxoid vly ml
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Cocalico Inc vly toxin
(A) Human erythrocytes were exposed to varying concentrations <t>of</t> <t>purified</t> recombinant <t>VLY</t> for 30 min. Cells were pelleted, and hemoglobin release was determined by OD 415 of the supernatant. Values were normalized to 100% lysis. When indicated, VLY was preincubated with pre-bleed (VLY+PB) or immune serum (VLY+IS) diluted 1∶50 for 30 min prior to use in the assay. (B) Erythrocytes were exposed to VLY (500 ng/ml), VLY+PB, or serial dilutions of VLY+IS (P<0.01 for VLY+PB versus all VLY+IS dilutions).
Vly Toxin, supplied by Cocalico Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Federation of European Neuroscience Societies sandwich elisa for vly quantification
(A) Human erythrocytes were exposed to varying concentrations <t>of</t> <t>purified</t> recombinant <t>VLY</t> for 30 min. Cells were pelleted, and hemoglobin release was determined by OD 415 of the supernatant. Values were normalized to 100% lysis. When indicated, VLY was preincubated with pre-bleed (VLY+PB) or immune serum (VLY+IS) diluted 1∶50 for 30 min prior to use in the assay. (B) Erythrocytes were exposed to VLY (500 ng/ml), VLY+PB, or serial dilutions of VLY+IS (P<0.01 for VLY+PB versus all VLY+IS dilutions).
Sandwich Elisa For Vly Quantification, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Absolute Biotech Inc primary vly antibody 23a2
(A) Human erythrocytes were exposed to varying concentrations <t>of</t> <t>purified</t> recombinant <t>VLY</t> for 30 min. Cells were pelleted, and hemoglobin release was determined by OD 415 of the supernatant. Values were normalized to 100% lysis. When indicated, VLY was preincubated with pre-bleed (VLY+PB) or immune serum (VLY+IS) diluted 1∶50 for 30 min prior to use in the assay. (B) Erythrocytes were exposed to VLY (500 ng/ml), VLY+PB, or serial dilutions of VLY+IS (P<0.01 for VLY+PB versus all VLY+IS dilutions).
Primary Vly Antibody 23a2, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cocalico Inc purified vly toxin
(A) Human erythrocytes were exposed to varying concentrations <t>of</t> <t>purified</t> recombinant <t>VLY</t> for 30 min. Cells were pelleted, and hemoglobin release was determined by OD 415 of the supernatant. Values were normalized to 100% lysis. When indicated, VLY was preincubated with pre-bleed (VLY+PB) or immune serum (VLY+IS) diluted 1∶50 for 30 min prior to use in the assay. (B) Erythrocytes were exposed to VLY (500 ng/ml), VLY+PB, or serial dilutions of VLY+IS (P<0.01 for VLY+PB versus all VLY+IS dilutions).
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Cocalico Inc recombinant purified vly toxin
(A) Human erythrocytes were exposed to varying concentrations <t>of</t> <t>purified</t> recombinant <t>VLY</t> for 30 min. Cells were pelleted, and hemoglobin release was determined by OD 415 of the supernatant. Values were normalized to 100% lysis. When indicated, VLY was preincubated with pre-bleed (VLY+PB) or immune serum (VLY+IS) diluted 1∶50 for 30 min prior to use in the assay. (B) Erythrocytes were exposed to VLY (500 ng/ml), VLY+PB, or serial dilutions of VLY+IS (P<0.01 for VLY+PB versus all VLY+IS dilutions).
Recombinant Purified Vly Toxin, supplied by Cocalico Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation mouse monoclonal anti-vly antibody
(A) Human erythrocytes were exposed to varying concentrations <t>of</t> <t>purified</t> recombinant <t>VLY</t> for 30 min. Cells were pelleted, and hemoglobin release was determined by OD 415 of the supernatant. Values were normalized to 100% lysis. When indicated, VLY was preincubated with pre-bleed (VLY+PB) or immune serum (VLY+IS) diluted 1∶50 for 30 min prior to use in the assay. (B) Erythrocytes were exposed to VLY (500 ng/ml), VLY+PB, or serial dilutions of VLY+IS (P<0.01 for VLY+PB versus all VLY+IS dilutions).
Mouse Monoclonal Anti Vly Antibody, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LoveYourself Inc trans-specific health centre
(A) Human erythrocytes were exposed to varying concentrations <t>of</t> <t>purified</t> recombinant <t>VLY</t> for 30 min. Cells were pelleted, and hemoglobin release was determined by OD 415 of the supernatant. Values were normalized to 100% lysis. When indicated, VLY was preincubated with pre-bleed (VLY+PB) or immune serum (VLY+IS) diluted 1∶50 for 30 min prior to use in the assay. (B) Erythrocytes were exposed to VLY (500 ng/ml), VLY+PB, or serial dilutions of VLY+IS (P<0.01 for VLY+PB versus all VLY+IS dilutions).
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Image Search Results


(A) Human erythrocytes were exposed to varying concentrations of purified recombinant VLY for 30 min. Cells were pelleted, and hemoglobin release was determined by OD 415 of the supernatant. Values were normalized to 100% lysis. When indicated, VLY was preincubated with pre-bleed (VLY+PB) or immune serum (VLY+IS) diluted 1∶50 for 30 min prior to use in the assay. (B) Erythrocytes were exposed to VLY (500 ng/ml), VLY+PB, or serial dilutions of VLY+IS (P<0.01 for VLY+PB versus all VLY+IS dilutions).

Journal: PLoS ONE

Article Title: Antibody-Based Detection and Inhibition of Vaginolysin, the Gardnerella vaginalis Cytolysin

doi: 10.1371/journal.pone.0005207

Figure Lengend Snippet: (A) Human erythrocytes were exposed to varying concentrations of purified recombinant VLY for 30 min. Cells were pelleted, and hemoglobin release was determined by OD 415 of the supernatant. Values were normalized to 100% lysis. When indicated, VLY was preincubated with pre-bleed (VLY+PB) or immune serum (VLY+IS) diluted 1∶50 for 30 min prior to use in the assay. (B) Erythrocytes were exposed to VLY (500 ng/ml), VLY+PB, or serial dilutions of VLY+IS (P<0.01 for VLY+PB versus all VLY+IS dilutions).

Article Snippet: Purified VLY toxin was generated and submitted to Cocalico Biologicals (Reamstown, PA).

Techniques: Purification, Recombinant, Lysis